Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM - Procédés des milieux dispersés Access content directly
Conference Papers Year :

Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM

Abstract

These experimental data are concerning the optimization of freeze-drying cycles of a model type Casei probiotic bacteria with water based formulations using lactose and polymer polyvinylpyrolidone (PVP) as cry/lyo protectants. After a previous study devoted to the influence of the main freeze-drying parameters (freezing rates; shelf temperature; total gas pressure) on the bacteria survival ratios, this paper reports some morphological data obtained by scanning electron microscopy (SEM) with the final lyophilisates. The numerous and original SEM images realized with different formulations of water binary or ternary mixtures with fixed operating conditions proved to be very useful to explain and to understand the different bacteria survival ratios observed., Thus, with bacteria or micro-organisms systems, this powerful investigation method of characterization of lyophilisates morphology should be more largely used in the future for setting up the optimal formulations parameters (composition) and the connected operating physical parameters of freezedrying steps (freezing rate, shelf temperature, total gas pressure).
Fichier principal
Vignette du fichier
document_2022.pdf (1005.5 Ko) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03773966 , version 1 (09-09-2022)

Licence

Attribution - NonCommercial - NoDerivatives

Identifiers

Cite

Séverine Crastes-Vessot, Ghania Degobert, Géraldine Agusti, Claudia Cogné, Julien Andrieu, et al.. Study and optimization of freeze-drying cycles of model probiotic: Morphology characterization of Lyophilisates by SEM. 22nd International Drying Symposium, Jun 2022, Worcester, United States. pp.1-9, ⟨10.55900/auksdudu⟩. ⟨hal-03773966⟩
41 View
47 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More