Conference Papers Year : 2018

Development of an integrated microfluidic algal biosensor for water pollution: tests on real samples

Abstract

In this work, we present an achieved fluorimeter used for water pollution detection. The principle of detection is based on the A-chlorophyll fluorescence measurement of microalgae, inserted by capillarity into low-cost and disposable xurography-based microfluidic chips. Three microalgae populations were used to develop the biosensor: Chlorella vulgaris (Cv), Pseudokirchneriella subcapitata (Ps), and Chlamydomonas reinhardtii (Cr). The algal concentration and the light intensity were optimized beforehand to calibrate the biosensor sensitivity with a pesticide (Diuron). Finally, the biosensor was employed for the pollution level measurement of 10 aqueous urban polluted samples (7 urban wet-weather discharges and 3 wastewater) in order to prove its reliability, reproducibility, and performance. Results are very promising, and leads to more complex chips in the future.

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Dates and versions

hal-01958235 , version 1 (17-12-2018)

Identifiers

  • HAL Id : hal-01958235 , version 1

Cite

Antoine Gosset, Louis Renaud, Claude Durrieu, Pauline Barbe, Rémy Bayard, et al.. Development of an integrated microfluidic algal biosensor for water pollution: tests on real samples. 11èmes journées Maghreb-Europe Materiaux et Applications aux DIspositifs et CApteurs (MADICA 2018), Nov 2018, Madhia, Tunisia. ⟨hal-01958235⟩
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