Efficient cytosolic delivery of luminescent lanthanide bioprobes in live cells for two-photon microscopy - Université de Lyon
Journal Articles Chemical Science Year : 2024

Efficient cytosolic delivery of luminescent lanthanide bioprobes in live cells for two-photon microscopy

Daouda Ndiaye
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  • PersonId : 1245128
  • IdRef : 268879788
Ji-Hyung Choi
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  • PersonId : 1285911
  • IdRef : 262657627
Lucile Bridou
Guillaume Micouin
Akos Banyasz
Olivier Maury

Abstract

Lanthanide(III) (Ln3+) complexes have desirable photophysical properties for optical bioimaging. However, despite their advantages over organic dyes, their use for microscopy imaging is limited by the high-energy UV excitation they require and their poor ability to cross the cell membrane and reach the cytosol. Here we describe a novel family of lanthanide-based luminescent probes, termed dTAT[Ln·L], based on (i) a DOTA-like chelator with a picolinate moiety, (ii) a two-photon absorbing antenna to shift the excitation to the near infrared and (ii) a dimeric TAT cell-penetrating peptide for cytosolic delivery. Several Tb3+ and Eu3+ probes were prepared and characterized. Two-photon microscopy of live cells was attempted using a commercial microscope with the three probes showing the highest quantum yields (>0.15). A diffuse Ln3+ emission was detected in most cells, which is characteristic of cytosolic delivery of the Ln3+ complex. The cytotoxicity of these three probes was evaluated and the IC50 ranged from 7 μM to >50 μM. The addition of a single positive or negative charge to the antenna of the most cytotoxic compound was sufficient to lower significantly or suppress its toxicity under the conditions used for two-photon microscopy. Therefore, the design reported here provides excellent lanthanide-based probes for two-photon microscopy of living cells.
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Dates and versions

hal-04608545 , version 1 (15-10-2024)

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Kyangwi Malikidogo, Thibault Charnay, Daouda Ndiaye, Ji-Hyung Choi, Lucile Bridou, et al.. Efficient cytosolic delivery of luminescent lanthanide bioprobes in live cells for two-photon microscopy. Chemical Science, 2024, 15 (25), pp.9694-9702. ⟨10.1039/d4sc00896k⟩. ⟨hal-04608545⟩
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