Ribozyme Chemistry: to be or not to be under high pressure - Biosynthèse des Signaux Peptidiques Access content directly
Journal Articles Chemical Reviews Year : 2019

Ribozyme Chemistry: to be or not to be under high pressure


The use of high hydrostatic pressure to investigate structure-function relationships in biomacromolecules in solution provides precise information about conformational changes and variations of the interactions between these macromolecules and the solvent , as well as the volume changes associated with their activity. The complementary use of osmotic pressure reveals quantitatively the extent and direction of the water exchanges between the macromolecules and the solvent and the number of water molecules involved in these exchanges. In this review, the chemistry of ribozymes and the influence of pressure is described. In the case of the hairpin ribozyme, pressure slowed down the self-cleavage reaction on the basis that the formation of the transition state involves a positive ∆V = of activation and the release of 78±4 water molecules. The self-cleaving activity of the hammerhead ribozyme is also slowed down by pressure on the basis of kinetic parameters and ∆Vs comparable to those of the hairpin ribozymes.
Fichier principal
Vignette du fichier
High-Pressure-Ribozyme-Chemistry.pdf (10.54 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02317015 , version 1 (17-10-2019)
hal-02317015 , version 2 (11-02-2020)



Marie Christine Maurel, Fabrice Leclerc, Guy Hervé. Ribozyme Chemistry: to be or not to be under high pressure. Chemical Reviews, 2019, 120 (11), pp.4898-4918. ⟨10.1021/acs.chemrev.9b00457⟩. ⟨hal-02317015v2⟩
258 View
494 Download



Gmail Mastodon Facebook X LinkedIn More